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Fatty Acid Binding by Soybean Lipoxygenase-1

Soybean Lipoxygenase-1

Two different research groups have published three papers describing the crystal structure of soybean lipoxygenase-1. All of these structures are of the inactive ferrous form of the enzyme. The references are as follows:

  1. Boyington, J. C., Gaffney, B. J., & Amzel, L. M. (1993). “The three-dimensional structure of an arachidonic acid 15-lipoxygenase”. Science, 260, 1482-1486.
  2. Minor, W., Steczko, J., Bolin, J. T., Otwinowski, Z., & Axelrod, B. (1993). “Crystallographic determination of the active site iron and its ligands in soybean lipoxygenase-1”. Biochemistry, 32, 6320-6323.
  3. Minor, W., Steczko, J., Stec, B., Otwinowski, Z., Bolin, J. T., Walter, R., & Axelrod, B. (1996). “Crystal structure of soybean lipoxygenase L-1 at 1.4 Å resolution”. Biochemistry, 35, 10687-10701.

The pictures that follows were based on coordinates of the 1.4 Å structure (1996 paper). These were kindly provided by Bernard Axelrod prior to publication of the structure. Updated coordinates have been deposited with the Brookhaven Protein Structure Databank.

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